Histopathological analysis of Colossoma macropomum liver, treated with bioproducts used by controlling parasites

Heidiane Nascimento  Feitosa; Klaus Casaro Saturnino; Raul  Dirceu Pazdiora; Henrique Momo  Ziemniczak; Simone  Paiva Medeiros; Bruna Rafaela  Caetano Nunes Pazdiora

doi:10.54502/msuceva.v2n1a11

Autores/as

Heidiane Nascimento Feitosa Prefeitura Municipal de Buritis: Buritis, Rondônia, Brasil https://orcid.org/0000-0002-7497-7602
Klaus Casaro Saturnino Universidade Federal de Goiás: Jataí, Goiás, Brazil https://orcid.org/0000-0001-8493-8669
Raul Dirceu Pazdiora Universidade Federal de Rondônia: Rolim de Moura, Rondônia, Brazil https://orcid.org/0000-0002-5495-4737
Henrique Momo Ziemniczak Universidade Estadual de Londrina: Londrina, PR, Brazil https://orcid.org/0000-0002-7198-8939
Simone Paiva Medeiros Universidade Federal de Rondônia campus Presidente Médici, Rondônia State, Brazil https://orcid.org/0000-0003-4647-7851
Bruna Rafaela Caetano Nunes Pazdiora Universidade Federal de Rondônia campus Presidente Médici, Rondônia State, Brazil https://orcid.org/0000-0002-5262-0514

DOI:

https://doi.org/10.54502/msuceva.v2n1a11

Palabras clave:

Acanthocephala, Amazon fish, extract of garlic, Neoechinorhynchus buttnerae, organic acids

Resumen

Possible histological changes in the liver of Colossoma macropomum Cuvier, 1816 (tambaqui) generated by the use of products incorporated into the diet as a control method of Neoechinorhynchus buttnerae Golvan, 1956 were evaluated. 1036 juveniles tambaqui with 77±3.0g of average weight were used, maintained in 28 polyethylene tanks of 1000L, with constant aeration and water recirculation system, using UV filter. The experimental units (30 fish per experimental unit) were distributed into four treatments, with seven replicates. The products, incorporated in fish oil, were added to the feed, according to the treatments: control group with commercial feed (Nutrizon Karino 36% GW) (A); extract of garlic (B); organic acids (C) and extract of garlic + organic acids (D). A parasitic and histopathological evaluation was performed at time zero (T0). During the experiment, two samples were collected, with an interval of five weeks, evaluating 15 fish per experimental unit. At the end, 63 liver samples for histological analysis were collected. The evaluated animals presented the expected growth for the species. 87.2% of efficacy was obtained with the association extract of garlic + organic acids. Hepatic morphological lesions compatible with toxicological processes were not observed, indicating a low toxicity for tested concentrations.

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Biografía del autor/a

Heidiane Nascimento Feitosa, Prefeitura Municipal de Buritis: Buritis, Rondônia, Brasil

Graduated in Fisheries Engineering from the Federal University of Rondônia (2018). During graduation, she was a volunteer in research carried out at CESA (Experimental Animal Health Center) and LCA (Laboratory of Environmental Sciences) in parasitology and fish health from 2015 to 2018. She is currently Coordinator of Fish Farming Programs at the Municipality of Buritis-RO, Brazil. She has experience in the area of Fish Farming, Rural Extension, Research Projects for Rural Sustainable Development and Preparation of Projects for Rural Credit.

Klaus Casaro Saturnino, Universidade Federal de Goiás: Jataí, Goiás, Brazil

Graduated in Veterinary Medicine from the State University of Londrina (2001), Master's from the Federal University of Mato Grosso do Sul (2009) and doctorate from the same HEI (2016) with the Postgraduate Program in Animal Science. He is a professor at the Federal University of Jataí, Brazil along with the Veterinary Medicine, teaching the subjects of General Pathology and Special Veterinary Pathology. He has experience in the areas of General Biology, with emphasis on Pathology (General Pathology and Special Pathology), Anatomy and Diagnostic Imaging and Ichthyopathology. He currently holds the position of coordinator of the Veterinary Medicine Course and coordinator of the Veterinary Pathology and Parasitology Laboratory at the Federal University of Jataí, Brazil.

Raul Dirceu Pazdiora, Universidade Federal de Rondônia: Rolim de Moura, Rondônia, Brazil

Associate Professor Level III at the Federal University of Rondônia- Department of Zootechnics, Presidente Médici Campus, Brazil. He has a degree in Veterinary Medicine from the Federal University of Santa Maria (2005), a Master's in Animal Science (Animal Production) from the same institution (2008) and a Doctorate in Animal Science (Animal Production) from the São Paulo State University. Júlio de Mesquita (UNESP), Jaboticabal campus (2011). He has experience in Veterinary Medicine and Zootechnics, with an emphasis on Animal Production-Beef and Dairy Cattle, working mainly on the following topics: feed evaluation, ruminant nutrition, genetic improvement and animal management.

Henrique Momo Ziemniczak, Universidade Estadual de Londrina: Londrina, PR, Brazil

Graduated in Veterinary Medicine at the Federal University of Rondônia - UNIR, Campus Rolim de Moura. Lato Post-Graduation in Teaching in Higher Education at Faculdade Única Educacional Ltda. Master in Animal Production at the Postgraduate Program in Animal Science at the Federal University of Grande Dourados -UFGD. Doctoral student in Animal Science at the State University of Londrina - UEL. He works in the fish farming area focused on ichthyopathology (bacteria, mycotoxins, parasites), with an emphasis on pirarucu, tambaqui, matrinxã and tilapia species at the Laboratory of Fish Bacteriology - LABBEP.

Simone Paiva Medeiros, Universidade Federal de Rondônia campus Presidente Médici, Rondônia State, Brazil

Graduated in Environmental Management from Universidade Norte do Paraná UNOPAR, Brazil (2013). Graduated in Fisheries Engineering from the Federal University of Rondônia, Brazil (2018). Master in Aquaculture and Sustainable Development from the Federal University of Paraná – UFPR, Brazil. Specialization in MBA in Agribusiness (2021), worked as a volunteer in research projects with a partnership company Nutrizon Alimentos / Neovia linked to the Federal University of Rondônia, Brazil developing research, CESA (Experimental Center for Animal Health) on the following topics: management, health of organism’s aquatic organisms with an emphasis on ectoparasites and endoparasites. She worked at Pisciculture Nova Esperança as a field supervisor with an emphasis on management and health.

Bruna Rafaela Caetano Nunes Pazdiora, Universidade Federal de Rondônia campus Presidente Médici, Rondônia State, Brazil

Doctor in Agricultural Sciences with emphasis on Tropical Animal Production, by the Universidad Nacional de Colombia (2014); Doctor in Animal Science, concentration area: Animal Production, by the State University of Maringá, Brazil (revalidation of the diploma obtained abroad) (2014). Specialization in Higher Education Didactics from the Faculty of Biomedical Sciences of Cacoal, Brazil (2008). Degree in Animal Science from the State University of Maringá, Brazil (2004). She is currently a professor at the Federal University of Rondônia (UNIR), Presidente Médici campus, Brazil. She has experience in Animal Science, working mainly on the following topics: Welfare and management of monogastric animals, focusing on equideoculture and fish farming.

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